الفهرس يوجد فقط 14 صفحة متاحة للعرض العام
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المستخلص
Minimal residual disease refers to the postremission persistence of
leukemia that cannot be detected by histomorphologic assessment.
Techniques for MRD detection can be classified based on the type
of cell marker used to identify the malignant cell clone, as follows:
Morphology, cell culture techniques that evaluate the pattern of in vitro
colony growth, cytogenetic characteristic of blast cells evaluated either
by conventional chromosome analysis or by fluorescence in situ
hybridization (FISH), the immunophenotypic profile of blast cells
assessed by multiparametric flow cytometry, DNA sequences analyzed
by polymerase chain reaction (PCR), Gene expression pattern of genes
relevant to tumor development assessed by microarray methodology.
The efficacy and applicability of the different methodological
approaches that are available for the detection of MRD depend on
three main features :
-Specificity: discrimination between malignant and normal cells,
without false-negative and false-positive results.
-Sensitivity: the detection limit of a MRD technique must be at least 10¯³