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Abstract
Entamoeba histolytica and Giardia lamblia are very important causes of
diarrhea due to parasitic diseases. Their prevalence varies in different areas of the world. In the present study, a trial to evaluate the role of both organisms in cases of diarrhea among children and adults was conducted. An enzyme-linked
immunosorbent assay (ELISA) was used for detection of Entamoeba species and
compared it with microscopic examination.
Regarding Entamoeba direct wet
examination was positive in 21.1% of samples, concentration method was positive in 22.2% and ELISA was positive in 16.7%. Sensitivity of ELISA was found to be 72.2% and specificity was found to be 97.2 %. So, it was found that ELISA is a more specific test than a sensitive test.
Direct examination for Giardia lamblia gave positive results in 12.4% of samples and 14.4% by concentration method. ELISA test is expensive compared to the conventional microscopic examination, but, it yields objective results and do not require experienced microscopists so can be recommended for survey studies.