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Abstract
Recent techniques for differentiation between pathogenic and non pathogenic avian P. multocida micro-organism.
This study was carried out to:
1- Investigate the prevalence of P. multocida serotypes among some
broiler, layer and breeder chicken flocks in Egypt.
2- Identify the isolated P. multocida biochemically and serologically.
3- Study the antibiotic sensitivity of isolated strains to the most available
chemotherapeutic agents in vitro.
4- Determine the pathogenicity of isolated strains.
5- Study the plasmid profiles of the isolated P. multocida strains.
The obtained results could be summarized as follows:
1- Thirty two P. multocida isolees could be obtained from 840 samples
(taken from 161 flocks including 39 broilers, 47 layers and 75 breeder
flocks).
2- The percentages of P. multocida isolation in layers and breeders were
29.7% and 24%, respectively. The morbidity ranged from 12-17 % in
layers and 8-12 % in breeders but the mortality rate was 3-6 % and 4-7%
in layers and breeders, respectively.
3- The Thirty two P. multocida isolates could be typed:
a- According to capsular typing by Indirect Haemagglutinationtest: 30 isolates could be typed as capsular (A), 1 typed as capsular
(D) and 1 isolate was untypable.