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Abstract
Brucellosis not only a zoonotic disease , but also causes heavy
economic losses in animal industry, that give the high importance of
its control which depend mainly on accurate serological diagnosis and
vaccination.
Among all serological tests that had been developed , cELISA
could overcome the problem of cross reaction that occurred from other
bacteria sharing identical epitope , beside being rapid and highly
sensitive.
I
This assay performed by using mono clonal antibody against A
and M epitopes from lipopolysaccharides of S 19 and Rev 1
respectively , as serological responses following infection or
vaccination directed predominantly against the lipopolysaccahrides.
. The prepared lipopolysaccharides have been characterized by