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Abstract A total of 185 samples were collected from 93 birds from different ostrich farms at Sharkia, Ismailia and Alexandria province, Egypt, including 46 cloacal swabs and 96 each of lung and liver ,samples. E. coli was isolated by percentage of 42, 29 and 200/0 from Ismailia, Alexandria and Sharkia provinces respectively with a total incidence of 54.60/0. Meanwhile, Salmonellas were isolated by a percentage of 1.4% from Ismailia province while it was failed to be detected in Alexandria and Sharkia provinces. Overall incidence of other members of Entrobactericeae and Pseudomonas species were isolated with a frequency ranged from 0.0 to 15.20/0. Regarding the serotyped E. coli strains in relation to a total of 31 E. coli isolates. It was found that 055, 078, 0125, 0127 and untypable were identified with the percentage of 32.3, 22.6, 3.2, 16.1, 25.8, respectively. While Salmonella isolates in total of 3 isolates and the obtained results were 2 isolates belonged to Salmonella typhimurium, |