الفهرس 
arabic summaryOnly 14 pages are availabe for public view
 |  | from | 141 |  |  |
| | | from | 141 | | |
Abstract
Three diagnostic tests were evaluated for diagnosis of paratuberculosis including, faecal microscopy, conventional faecal culture, and polymerase chain reaction. In this respect, 145 faecal samples were collected (from four cow herds) and examined for presence of the causative agent of Johne’s disease, M. paratitherculosis. The cow herds were as following: 1
The first herd: is a private herd with 35 adult cows. The faecal samples were collected for routine bacteriological examination.
2- The second herd: it is a University herd with 42 animals. The animals were certified-paratuberculosis-free herd and kept under strict quarantine measures. The samples were collected for routine examination.
3- The third herd: is a private one with 37 cows. This herd had previous history of paratuberculosis.
4- The fourth herd: is the herd of faculty of agriculture, Assiut University. 31 faecal specimens were collected from cows suffering from diarrhea and/or emaciation.
The samples were examined by the three diagnostic tests and the obtained results were evaluated for diagnosis of Johne’s disease. The recorded results were fully discussed and briefly as
Faecal microscopy (faeccal smear)
The faecal specimens were prepared for Ziehi-Neelsen staining characteristics. The obtained results revealed that the faecal specimens containing acid-fast stained bacilli were 14.3; 16.7, 21.6, and 12.9 % for the first, second, third, and forth herd, respectively. The overall prevalence rate by this test was 16.6%.