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Abstract
This in vitro study was conducted to study the effect of pH of the material, number of applications and citric acid challenge on dentinal tubule occlusion using 3% potassium mono-hydrogen oxalate desensitizing agent.
Forty freshly extracted molar teeth were selected. The teeth were splitted mesio-distally, slightly grounded and wet polished to prepare 80 buccal and lingual halves with simulated hypersensitive radicular region.
The eighty prepared specimens were divided into two main equal groups (40 specimens each), according to the pH of the desensitizing agents used (D) where in D1 mono-potassium mono-hydrogen oxalate with low pH (2.5) was used, while in D2 mono-potassium mono-hydrogen oxalate with higher pH (4.5) was used. Each group was further subdivided into two equal subgroups (20 specimens each) according to the number of applications to which the specimens were exposed (A), where in A1 the specimens were subjected to a single material application and in A2 the specimens were subjected to a double material application. Each subgroup was divided into two smaller classes (10 specimens each) according to whether the treated samples were subjected to 6% citric acid after termination of the treatment or not (C), where in C1 the specimens were not subjected to citric acid challenge, while in C2 the specimens were subjected to 6% citric acid.
In each prepared specimen, one half of the radicular dentin was left untreated and considered as a control, while the other half was treated according to the specific assigned group. SEM examination was done for the dentin specimens under different magnifications and photomicrographs were taken for both control and treated sides.