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Abstract
being convinced with this idea,this study was carried out to give a spot light on the morphopathological alterations evoked by acute and chronic toxicosis of acrylamide in nile tilapia in nile tilapia in addition to some biochemical analysis to evaluate the hepatotoxic affect of acrylamide and study its effect on the entioxidative status.
For this purpose, a total number of 246 apparently healthy Nile tilapia were divided into three experiments; one hundred and twenty six fish were classified into 7 equal groups namely. 1,2,3,4,5,6 and 7 which were given daily 0,12.5,25,50,100,200 and 400 mg/L acrylamide, respectively to determine the 96hrs LC50. The 96hrs LC50 of acrylamide in Nile tilapia was 100 mg/L.
Regarding acute toxicity experiment; sixty fish were classified into two equal groups, control group and treated group that was exposed to 50 mg/L (½ LC50) renewed daily for 7 days. Sixty fish divided into equal control and treated group that exposed to 10 mg/L (1/10 LC50) for 8 weeks renewed every three days were used in chronic toxicity experiment.
Gills, hepatopancreas, brain, kidney, spleen and GIT were collected daily in the acute toxicity experiment to describe the associated pathological lesions, while weekly collection of the pervious mentioned organs and blood was carried in the chronic toxicity experiment to describe the pathological changes with determination of the glutathione level in gills, liver and brain and measurement of the levels of serum AST, ALT and albumin.
Both acute and chronic acrylamide toxicity in Nile tilapia produced nearly the same effect, but higher dose resulted in more damage, earlier onset and quicker progressive effect.
Clinically, the affected fish in both experiment suffered from respiratory and nervous manifestations. Grossly, there were congestion of the gills and the internal organs especially the hepatopancreas.