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Abstract In the present study a total of 200 samples were collected from apparently healthy and diseased human, animals and poultry which revealed a total of 82 yeast isolates are subjected for antifungal sensitivity tests. Eighty two isolated yeast obtained from animals, poultry and human were identified into Candida albicans (54), Candida parapsilosis (2), Candida krusei (1), Malassezia pachydermitis (6), Malassezia furfur (5), Trichosporon beigelii (2) and (12) Rhodotorula rubra. All isolated yeast were subjected for disc antimycotic susceptibility tests using:fluconazole, clotrimazole, ketoconazole, itraconazoleamphotericin B and nystatinThe results revealed the following: Candida albicans isolated from poultry showed higher susceptibility to nystatin followed by amphotericin B, while those isolated from cattle were most sensitive to clotrimazole followed by nystatin. Candida albicans isolated from human samples were most sensitive to ketconazole as first choice followed by clotrimazole. Candida parapsilosis isolated from human showed higher susceptibility to itraconazole followed by clotrimazole. Candida krusei isolated from human showed higher susceptibility to fluconazole followed by clotrimazole and nystatin. Trichosporon beigelii isolated from human showed higher susceptibility to itraconazole followed by nystatin. Malassezia furfur isolated from human showed higher susceptibility to ketconazole followed by fluconazole. Malassezia pachydermitis isolated from dogs showed higher susceptibility to amphotericin B followed by nystatin. Rhodotorula rubra isolated from human showed higher susceptibility to amphotericin B followed by nystatin, while |