الفهرس | Only 14 pages are availabe for public view |
Abstract In the present study, we determined the in vitro cytochrome P450- mediated biotransformation activities for five pharmacologically important substrates in male leghorn chickens and beagle dogs to evaluate the liver catalytic activities in normal chicken and compare it with those of dogs. According to our results, the chicken total cytochrome P450 content (0.296±0.019 nmol/mg protein) was almost similar to the values reported in humans, cats, pigs, trout fish, snakes, and frogs. On the other hand, chicken total cytochrome P450 content was lower than levels reported for most of the other species including the beagle dog (l.114±0.099 nmol/mg protein). We also investigated the effects of lipopolysaccharide on the cytochrome P450-mediated activities and antipyrine pharmacokinetic to evaluate the drug catalytic activity in diseased chicken. After injection of lipopolysaccharide (50 mg/kg b. w.) to chicken, total hepatic microsomal cytochrome P450 content was decreased by about 20%, antipyrine elimination rat constant was also decreased by about 44%, while antipyrine plasma half-life was significantly increased by about 77%. |