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Abstract
locations in Suez Canal area were examined for brucellosis.
I .
Blood samples of all animals were serologically examined using
four serologically tests namely Buffered Acidified Plate test, Rose Bengal
test, Tube Agglutination test and Rivanol test.
The results revealed a prevalent of 17 (0.98%) in sheep 8 (0.94%)
In goat using BAPT, 17 (1.55%) in sheep and 6 (0.71 %) in goat using
RBT, 20 (1.15%) in sheep and 7 (0.94%) in goat using TAT and 14
(0.80%) in sheep and 8 (0.94%) in goat using Rivanol test.
Sensitivity and specificity and estimated f lse positive results were
calculated. In sheep Buffered acidified plate antigen test showed
sensitivity 94.1 %, specificity 99.94% and estimated false positive 0.06%.
~-’:.’•’,7
Rose Bengal Test showed sensitivity 94.1 %, specificity 99.94% and
estimated false positive 0.06%.
Tube agglutination test showed sensitivity 94.11 %, specificity
99.79% and estimated false positive 0.23%.
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Rivanol Test showed sensitivity 79.47% and specificity 99.94%
and estimated false positive 0.06%
In goats Buffered acidified plate anti gent test showed sensitivity
100% specificity 99.88% and estimated false positive 0.12%.
’~-~”’}’
Rose Bengal test showed sensitivity 75% specificity 100% and
estimated false positive Zero.
Tube agglutination test showed sensitivity 87.5%, specificity 100%
and estimated false positive Zero.
Rivanol Te~t showed sensitivity 100% specificity 99.88% and
estimated false positive 0.12%.
Brucella orgamsms could be isolated from tissue specimen of 2
slaughtered sera positive ewes and one milk sample.
Biotyping of 3 Brucella isolates, obtained in this study revealed Hr.
melitensis biovar 3.
The obtained results indicated the wide prevalence of Brucella
infection in sheep & goats in Suez Canal area. The matter whicli ... c.
necessitates organization of a national programme for control of brucella
infection in sheep & goats.
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