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Abstract
Mastitis is the most prevalent production disease in dairy herds. Subclinical
mastitis although it dose not lead to visible changes in the milk or udder, it is more
important economically than clinical mastitis due to higher prevalence, associated
with decrease in milk yield and altered potential quality and physiochemical
properties of milk so more attention has been focused for the diagnosis of subclinical
mastitis.
Our study topics include:
1- Study the frequency and severity of mastitis among the examined cows and
buffaloes, by using California mastitis test (CMT).
2-Laboratory measurement of somatic cell counts by means of Milk Scan Apparatus
(Milk Soma Counter) and study its correlation with incidence of subclinical mastitis
and isolated bacteria.
3- Bacteriological examination of quarter milk sample for isolation and identification
of causative organisms.
4- Study the immunological role during mastitis with special refrence to Lysozyme,
Immunoglobulin A.
The CMT was done to detect subclinical mastitis ,examination of 1068 and 480
cows and buffaloes reported that the subclinical mastitis constitute 57.3% and 66.2%
respectively. While the prevalence rates of clinical mastitis were 14.6% and 2.5%
among the tested cows and buffaloes.
The severity of mastitis was investigated by detecting the percentage of subclinical
mastitis in relation to the number of quarter milk samples. The severity of mastitis
was high in subclinical mastitis with score of +++ (44.25% and 42.9%), and ++
(12.2% and 18.1%), but a lower percentage (10.5% and6.8%) was in subclinical
mastitis with a score of + among the examined cows and buffaloes respectively.
In the present study, the stage of lactation may have an effect on udder
susceptibility to subclinical mastitis. Thus 39.2% and 44.3% in subclinical mastitic
udder were obtained from cows and buffaloes respectively during the early stage of
lactation.
S.aureus was the most prevalent species isolated from the tested cows and
buffaloes.Coagulase Negative Staphylococci (CNS),S.agalactiae and E.coli were
isolated either single or mixed infection from the examined samples in different
percentage.