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Abstract
Bovine Rotavirus (BRV) is a major cause of acute gastroenteritis in newborn calves throughout the world causing economic losses to beef and dairy industries. Neomycin B, an aminoglycoside antibiotic has been shown to inhibit Human immunodeficiency virus and Herpes simplex virus replication. The current study was carried out to assess the effect of neomycin B on rotavirus replication in-vitro on Rhesus monkey kidney fetal cells (MA-104) and in-vivo (in mice) for controlling Rotavirus gastroenteritis. The first approach of this work was to demonstrate the effect of neomycin B on BRV replication in MA-104 cells using IF, plaque assay and flow cytometry. In optimization of plaque assay of BRV, incorporation of 5 µg trypsin/ ml of the overlay (equal volume of 2x MEM and seaplaque agar 1.6%) was found to be necessary for NCDV plaque formation. .he cytotoxicity of neomycin was tested using MTT assay measuring metabolic activity of cells. The CC50 value for neomycin which is the reduction in viable cell by 50% is 30mM. Neomycin interfered with the development of BRV CPE. Neomycin concentrations at 5, 7.5 and 10 mM were able to reduce the CPE of BRV by 25%, 58.33% and 91.67% respectively.