الفهرس 
Chapter I : IntroductionOnly 14 pages are availabe for public view
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Abstract
This research aimed to isolate and recognize CPD virus in sheep and goat herds
and the research investigate the following:
1- The affected sheep and goats show clinical signs like nodules, pustules, scarsand crusts which are obviously appeared on lips, gums, mouth commisures and
eyelids.2- Samples from these affected animals were collected in order to isolate andrecognize the CPD virus with normal serological and non serological means. 3- Preliminary identification of the CPD virus antigen using electron microscopic
examination for skin samples which give characteristic appearance of the virus
(ovoid in shape, with rounded ends and characteristic ball of wool appearance).
4- Trials for isolation of CPD virus:
The prepared samples from skin lesions were inoculated in emberyonated
chicken eggs by the CAM route which showed thickening, oedema and
hemorrhage of CAMs tissue.
5- Titration of CPD virus isolates revealed that the titer of collected sheep
isolates was 5 log10 EID50 / ml while the titer of collected goat’s isolates was
4log10 EID50 / ml .
6- The inoculated CAMs were harvested and AGPT and IFAT tests were done
which gave positive results for viral antigen.
7- PCR test was done using known primer on harvested samples from CAM and
tissue samples. Amplification and running of characteristic fragments of CPD
viral DNA on Ethidium Bromide stained Agarose Gel then staining of DNA gave
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typical results in many segments on DNA fragment which indicate that the given
sample contain CPD viral antigen.
8- characterization and identification of CPD virus revealed that:
- The isolated virus has no haemagglutinating property to avian RBCs of
(chicken, duck, goose, turkey and pigeon RBCs).
- The isolated virus has no haemagglutinating property to mammalian RBCs of
(sheep, goat, donkey, horse, cattle and buffalo RBCs).
- The isolated virus has haemagglutinating property to rabbit RBCs
- The haemagglutination inhibition was applied and gave positive results in
form of button shape pattern.
from the previous we can conclude that the isolated virus from affected
animals (sheep and goats) assumed to be CPD virus as confirmed by AGPT,
IFAT and PCR.