الفهرس يوجد فقط 14 صفحة متاحة للعرض العام
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المستخلص
Samples were collected from birds in a poultry Flock at Al-Sharkia Governorate. Live birds showing from nervous and/or respiratory manifestation or samples were collected from freshly dead birds. Samples were prepared for inoculation in chicken embryos via allantoic sac . The collected allantoic fluid showing HA activity were identified using specific NDV antiserum. The Pathotyping were carried out by the criteria of MDT, ICPI and IVPI resultsindicated the virus to be a velogenic NDV strain. The complete genomic sequencing was carried out. The complete genomic sequence was submitted to the genbank under GenBank accession number FJ939313, and the nomenclature of the isolated virus was designed as NDV/chicken/Egypt/1/2005.The complete genome sequence of NDV/chicken/Egypt/1/2005 was
determined and compared with the sequence of NDV strains isolated from different parts of the world. The genome is 15,186 nucleotides (nt) long and consists of 6 genes in the order of 3’-N-P-M-F-HN-L-5’. The genome contains a 55-nt leader region at the 3’ end and a 114-nt trailer region at the 5’ end. The phylogenetic analysis showed that the NDV/chicken/Egypt/1/2005 is closely related with the NDV strains isolated in China. In addition, the sequence of the fusion protein cleavage site of NDV/chicken/Egypt/1/2005 strain was identical to that of the NDV strain recently isolated in Mali. To our knowledge this is the
only completely sequenced NDV strain isolated in Africa and Middle East Determination of complete genome sequences of additional NDV strains from Africa is necessary to understand the epidemiological features of NDV strains in Africa.