الفهرس 
INTRODUCTION AND AIM OF THE WORKOnly 14 pages are availabe for public view
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Abstract
1- introduce PCR technique in the laboratory of clinical pathology department at el-minia university hospital for diagnosis of HCV.
2- Optimize the detection of amplified product by comparison between gel detection and enzyme immune assay (ELA).
3-determine the prevalence of HCV-RNA between different groups as blood donor group, chronic renal failure group and chronic liver disease group.
4- study the correlation between liver enzymes and HCV-RNA by RT-PCR
5-determin the type of HCV genotyping in cases from USA and other cases from Egypt by using restriction fragment length polymorphism (RFLP).
Hepatitis C is a viral illness that affects the liver. It has a variable course that can lead to chronic hepatitis, cirrhosis, liver cell failure and HCC worldwide. HCV is spread by blood-to-blood contact. The course of illness may be adversely affected by various risk factors, bilharziasis especially who received tartar emetics, blood transfusion and sharing needle.
ELA-III should be the initial test for the diagnosis of hepatitis C. In low risk populations, HCV-RNA testing should be performed. but because of assay variability, HCV-PCR must be interpreted cautiously.
There is no correlation between levels of liver enzymes and HCV infection patients with HCV positive by RT-PCR and normal ALT levels should not be considered as healthy carriers because they are often viraemic.
HCV has many different genotypes closely to demographic distribution. HCV genotyping may provide useful prognostic information, but at present must be considered a research tool.