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Abstract Assisted reproductive technologies especially in vitro fertilization and intracytoplasmi sperm injection have a success rate of 15-40 % in the most controlled centers with the risk of multiple pregnancies and transmission degetic disorders to the result offsprings. from this point of view the era of pra_implantation genetic dignosis have been developed and established in almost all assited reproductive centers , aiming to transfer of less number of healthy embryos avoid multiple gestations and transmission of genetic dieases PGDis done by polar body biopsy from the oocytes blastomere biopsy from the eleavage embryo or the cells from of the blastocytes allthese meathods need IVFprocedre thoughts regarding biopsy procedure may affect embryo viability was raised by van steirteghem et al 1991 so the aim of this work was to assess the traumatic effect of blastomere biosy techniques on the embryo in the pre-implantation stage as regarding in vitro development such as division cavitation and blastocyst formation the study was carried out on in vitro fertilized mouse embryos at the third day post insemination after dividing the total number 44 embryos into two equal groups 22 embryos have been left without intervention and other 22 embryos have been subjected to biopsy procedure by acid tyrode,s method there is no significant statistical difference between the two groups approving that the biosy procedurehas no traumatic effect on biopsied embryos if done as described by ESHRE.PGD consortium 2000. |