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Abstract
low concentration solutions of DNA that are prone to DNA
degradation. Because of the difficulty in obtaining such samples,
and their potentially high value in forensic field (for examples:
identification, disputed paternity and inheritance cases). The
DNA storage for a time may be needed during judicial
proceeding, in cases which may be reopened or till availability
of PCR kit so it is critical that optimal preservative agent is
employed for DNA storage. The present study aimed to assess
the effect of Tris-EDTA (TE) and trehalose, as preservative
agents, on quality of DNA samples. The method of study was
storage of 2 known concentrations of human blood DNA (5-10
ng/ul) at 2 different temperatures ( -80°C, dried samples stored at
room temperature [RT]) and 2 preservative agents(TE, trehalose)
were used. The effectiveness of preservative agents was tested at
regular intervals (at set up, after 3 & 6 months) using PCR assay
of 757 base pair (bp) fragment of 18s ribosomal gene to evaluate
the quality of remaining DNA. The result showed that after 3
months, samples preserved with TE buffer had 43.8% PCR
success rate, while samples preserved with trehalose had 62.5%
PCR success rate. After 6 months, samples preserved with TE
buffer had 25 % PCR success rate, while samples preserved
with trehalose had 62.5% PCR success rate. The present study
showed that DNA quality was best preserved in the presence of
trehalose with significant quality loss occurred with TE after 6
months of storage.