الفهرس 
AcknowledgementOnly 14 pages are availabe for public view
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Abstract
Owing to the increase interest in chemical methods of analysis of antimicrobial agents, as it is examplified by the spectrophotometric analysis of chloramphenicol (B.P. 1973) and tetracyclines hydrochloride (U.S.P.XX 1980); the auther was concerned with searching for new chemical methods of analysis of some antimicrobial agents which may be of value for routine analysis.
The antimicrobial agents subjected to analysis are: 1- Chloramphenicol.
2- Tetracycline hydrochloride, oxytetracycline hydrochlooride, chlortetracycline hydrochloride and demeclocycline hydrochloride.
3- Triacetyloleandomycin.
The thesis comprises seven chapters Chapter I
This is a theortical part which includes discussion of the different methods of analysis of some antibiotics available in the literature.
Chapter 11 :
Explains the first adopted method for the spectrophhotometric determination of chloramphenicol using 4-nitro-
soantipyrine reagent. This method depends on the
reaction between 4-nitrosoantipyrine and p-nitrophenol
obtained by the hydrolysis product of chloramphe~icol by heating with 40~ sodium hydroxide solution. The
results obtained were precise and accurate. The method
is also applied for the analysis of chloramphenicol in
its pharmaceutical formulations.
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Comprises the second adopted method for the deter-
mination of five drugs using 2,3,5-triphenyltetrazolium
chloride reagent. The principle of the reaction between tetrazolium chloride and the drugs is due to the ability
of these compounds to reduce the tetrazolium salt to ~
highly coloured formazan derivative. Drugs determined
by this method were: tetracycline hydrochloride,
oxytetracycline hydrochloride, chlortetracycline hydroochloride, demeclocycline hydrochloride and triacetyl-
oleandomycin either in their pure forms or in their pharma-
ceutical formulations. The obtained results were found
accurate, precise and the method can be used for routine
analysis of these drugs.
Decribes the third method of analysis of tetracycline
hydrochloride and triacetyloleandomycin using some aromatic
aldehydes; vanillin, p-hydroxybenzaldehyde and anisaldehyde reagents. The procedure for the determination of these drugs required a previous dehydration of these drugs
using conc. sulphuric acid and then these drugs reacted with the different aldehydes used. The sensitivity of the reagents used are variable from one drug to another. Vanillin gave higher sensitivity for tetracycline hydroochloride and p-hydroxybenzaldehyde gave higher sensitivity for triacetyloleandomycin.
Results obtained were reliable, accurate and precise.
Includes the spectrophotometric determination of
some tetracyclines using hexamine/sulphuric acid reagent. The reaction is probably due to a condensation of the liberated formaldehyde with the dehydrated product of tetracyclines (under the influence of conc. sulphuric acid). Drugs determined by this method were : tetracycline hydroochloride, oxytetracycline hydrochloride, chlortetracycline hydrochloride and demeclocycline hydrochloride. The method proved to be precise, simple and time saving and can be used for routine analysis.
A spectrophotometric method was developed for the
analysis of tetracycline hydrochloride, oxytetracycline
hydrochloride, chlortetracycline hydrochloride and demecloocycline hydrochloride using a mixture of acetic acid, acetic anhydride (1:1) and conc. sulphuric acid. Good & concordant results were obtained with an error not exceedding + 1.35%. It can be recomended for routine analysis.
9Represents a comparative study between the official
B.P. 1973; U.S.P.XX 1980 and the different adopted methods for the assay of chloramphenicol, triacetyloleandomycin and some tetracyclines.
All the experimental results were tabulated in (44 ) tables and (43 ) Figurs.