الفهرس Only 14 pages are availabe for public view
 |  | from | 134 |  |  |
| | | from | 134 | | |
Abstract
H.pylori is a gram-negative, spiral-shaped,microaerophilic bacterium that infects the human gastric mucosa. Helicobacter pylori is one of the most common infective agents worldwide. It is an etiological agent of gastritis, peptic, and duodenal ulcer disease, and
infection with this organism is a recognized risk factor in the development of gastric mucosa associated lymphoid tissue lymphoma and adenocarcinoma In particular, this
organism has been categorized as a class1 carcinogen by the World Health Organization.
The diagnosis of H. pylori gastric infection can be conducted by using direct (invasive) or indirect (noninvasive) methods. Among the indirect methods, serology is a valuable tool for sero-epidemiological studies. As a result detection of circulating H. pylori antigens may be
of interest to be included in diagnosis of H. pylori infection.
The assay detected H. pylori antigen in serum samples of 83 infected patients with a sensitivity of 92%. Serum samples of 50 non-infected individuals were used to evaluate the specificity of the ELISA. The assay
showed that 46 non-infected individuals were negative by the ELISA, and this revealed 92% specificity. Statistical
characteristics of ELISA as a sensitive and simple diagnostic assay of H. pylori based on HpCA detection in
serum compared with standard culture revealed, positive predictive value of 95%; negative predictive value of 86
%;and efficiency 92 %. The diagnostic value of HpCA assessed by the area under the ROC curve. The area
under ROC curve of HpCA for discriminating infected patients from those non-infected and (p value) were 0.982(P<0.0001).