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Abstract
This study was done at the Oncology Diagnostic Unit- Faculty of Medicine, Ain Shams University, during the period
from 2006-2008 and included 46 patients with head and neck squamous cell carcinoma (HNSCC) and 16 patients with benign conditions.
This study aimed at investigating the impact of human papillomavirus (HPV) high-risk genotypes integration in head
and neck squamous cell carcinoma development that may improve understanding the causes of this disease in Egypt.
As HPV-16 E6 oncoprotein is able to induce the degradation of p53, and might have an effect on survivin transcription that renders survivin a potential candidate as a tumor marker. These considerations lead us to investigate the possible effects of the
high risk HPV genotypes on the expression of human survivin
gene.
The primary objective of this study was to evaluate the frequency of high risk genotypes HPV16E6 DNA and HPV18DNA, specifically in relation to antiapoptotic survivin gene overexpression as a biological marker in HNSCC.
Patients and Methods: Tissue specimens were obtained during surgery from 46 patients having different types of
HNSCC with their resection margins, and from 16 patients included as a control benign group. HPV-16 and HPV-18 were
detected using polymerase chain reaction while survivin-mRNA was detected by RT-PCR.
Results: HPV-16 was positive in 78.3% of the malignant group (of them 30.4% were positive margins) versus 68.8% in
the control one (P=0.32). On the other hand: HPV-18 shows 21.7% positivity in malignant tissues versus 25% in benign
group (P= 0.51) and 95.7% negative margins. Survivin was significantly higher in the malignant tissues, 45.7% with advanced stages than (P=0.00) 10.9%in their safety margins and was not detected in benign group 0%. Survival analysis showed no significant association between HPV16DNA and/or
HPV18 positivity and recurrence (or mortality) in the malignant tumors. No significant association between HPV genotypes and survivin expression in all studied groups was found.