الفهرس 
AcknowledgementOnly 14 pages are availabe for public view
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Abstract
The importance of tetracycline (TC) and >oxytetracycline (OTe) as widely used antimicrobial drugs initiated the analysts
to find a procedure appropriate for their assay in the pharmaaceutical formulations. Such problem has been approached in a
number of ingenious ways, appeared in hundreds of publications
during the last years. Nevertheless, all these published methods and their variants make the determination of these antimicrobial drugs in coexistence with their degradation products or pharmaaceutical formulation matrix tedious, lengthy and sometimes
impossible.
In this connection different methods of assay have been
here proposed. The spectrofluorometric methods using chelation with A13+ or Mg2+ was amended and rendered more selective specially in determining TC in the presence of anhydrotetracyycline (ATC) and vice versa. The use of the first derivative
spectrophotometric method enables to solve the same problem. Seperation by paper chromatagraphy with subsequent spectroophotometric measurement supported the results of the first derivative spectrophotometric method.
For the quantitative assay of TC and OTC in their dosage forms, the pH induced differential spectrophotometric methods (using zero-order and fiDst-order spectra) besides the above
mentioned methods have been utilized. The kinetic method, which is still of very limited application in pharmaceutical analysis, was applied in TO and OTO assay and based on their degradation rate with O.OIN HOl under certain experimental conditions.