الفهرس | Only 14 pages are availabe for public view |
Abstract The aim of this thesis is to evaluate the vaccine potential of s. mansoni antigens . In conclusion, one cDNA clone was isolated from gtll cercarial expression library by plaque lifts hybridization using an (Q-32P)probe of the fragment isolated by immunoscreening from Zap 7- days schistosomules expression library. The cDNA clone has a size of 1415 bp, with an open reading frame (ORF) of 155 A.A. , starting with the start codon (ATG) at position 11 and ending with the stop codon (TAA) at position 476. the coding sequence was subcloned into a prokaryotic expression system pGEX-4T-1 , then expressed and induced producing a fusion protein of 39 kDa molecular size. It was proved that the fusion protein is contaning glutathione S-transferase (GST) of 22 kDa molecular size and a recombinant protein of 17 kDa molecular size. Specific antibodies obtained agenst this fusion protein by the technique of microelution from nitrocellulose membrane recognized a protein of kDa molecular size in NP- 40 protein extract of S.mansoni adult worms , 5-days schistosmules 25-days schistosmules and soluble egg antigens (SEA), also recognized a protein of 23 kDa molecular size in the protein extract of adult worms , sercariae, 25-days schistosomules and SEA |