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Abstract
6.Summary:-
In this study two groups of ewes were taken the first group was (302) aborted cases and the second control group was (36) clinically healthy ewes. The incidence of abortion in the first group was high in age from up to 3 years to over 5 years (37.6%), while the old age ewes over 6 years the percent of positivists were 23.5% this due to these ewes may be carriers or have latent persistent with intermittent shedding of the organism. Clinically healthy ewes show incidence of infection of (2.7%), data from the clinical history of the first group and the character of lesions in aborted placentas suggest enzootic abortion.
Firstly demonstration of the pathogen where made by microscopic examination of smears made from the endocervical swabs taken from the aborted ewes stained with Giemsa the sensitivity, specificity, positive predictive value, negative predictive value of these tests were (12.5%), (99%), (100%), (76%). Isolation of the pathogen was done through inoculation of the samples into Vero cell line, both elementary and reticulate bodies were detected through staining of infected Vero cells with Gemsa`stain sensitivity, specificity, positive predictive value; negative predictive value was (92.5%),(100%), (100%), (97%). Detection of serum anti Chlamydial IgM by ELISA revealed sensitivity, specificity, positive predictive value, negative predictive value ,(97.5%) , (99%) , (100%),(98%).
Identification of isolated antigen (intracytoplasmic prenuclear Chlamydial inclusions) on Vero cells was done by IF technique using control positive serum against Chlamydophila psittaci and antispecies specific fluorescence conjugate; the sensitivity, specificity, positive predictive value, negative predictive value were all (100%).