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Abstract SUMMARY These studies were conducted at Nubaria Research Station,North Tharir, ARE duriogc1978- 1982 in order to investigate the effect of various levels of soil phosphorus application:. and some micronutrient tre~tmentswith two methodes of application on the quantitative and qualitative characters of alfalfa for three growing years. Treatments were as follows: Three soil phosphorus application levels 0,200, and 300 kq superphosphat (16% P205 l per fad during the soil preparation (broadcastlin the first year of establishment, were used. Soil application of micronutrients , * B 10kg per fad boric acid. * Zn 20kg per fad Zinc sulfate * Fe 30kg:perifad ferrous’sulfate. * Combinations of these micronutrients were applied each at the previously mentioned concentration * On the first year of establishment, micronutrients. were splited into two equal parts. The first part was applied at 45 days from SOWing the other part after the first cut. 194. • Folior application of micronutrients an aqua solution of 0.2% of B,zn,Fe, and their combinations at a rate of 400 liter per fad was used. Treatments were applied repeated after the first cut in shment. On the secand and third growing years, either soil or foliar micronutrient treatments and soil phosphorus application levels (broadcast) were applied o~ March of 1980 and 1981. 4 weeks from sowing and the first year of eatabli- Split-split plot design was used where methods of micronutrients application were assigned for the main plots, soil phosphorus applicatioft levels for the subplots, and the micronutrient treatments for the sub-sub plots. Eleven cuts were taken during each of the first and second growing years, and nine ..cut sdur ing the third growing year. The study included foroge and dry yield of each of the three growing years and the total yield of the three growing years. Some growth characters were measured using the second cut of spring, summer autummn, and winter for each of the three growing years. Chemical cQnstituents were analyzed for all cuts of each season (spring, summer, 195. autummn and winterlof the secand growing year. Results could be summarized as follows: 1- Total forage and dry yield was affected differently by vari ous soi 1 phosphor us application levels, micronutrient tretments, metheds of application, and the ” ., seasonal variations. 2- It was generally clear in three growing years and in most cuts that soil application of 200kg superphosphate per fad produced the highest forage and dry yield. However, the ext.a higher phosphorus rate (300kg superphosphate per fadldid not exhibit extra advantages in forag and dry yield. 3- Foliar application of the micronutrient treatments was more effective than soil application at zero and 200 kg superphosphate per fad as far as forage and dry yield are concerned. Whereas, at 300kg superphosphate per fad, different results were obtained where soil application of micrOAutrients was more productive in yield than foliar application. 4- The previously mentiened trend was more or less repeated in the three sucessive growing years. 5- In most cases, any o~ the applied micronutrient teatments either as a soil or folior application, with or without any of the applied soil phosphorus levels, produced a significant increase in alfalfa forage and dry yield as compared to the control. 6- The response of alfalfa dry yield to micronutrient treatments and methods of application at the first growing year was different than what was obtained at the second and third growing years. a- In the first growing year , the highest forage yield (27.60, .31.64 .ton per fad) ,was p.roduced by applying B+Fe+Zn to the soil, and foliar application of Fe + zn > b- However, the top dry yield was produced by the soil and foliar application of Zn Jnd Fe+Zn, respectively where the correspanding dry yield was 6.95 and 7.36 ton per fad. c- During the second and third growing years, soil application of B+Zn and foliar application of Fe + Zn respectivelYJproduced the highest forage yield which was 54.99 and 59.25 ton per fad in the second year The correspondin~ forag yield was 35.46 and 36.59 ton ppthird growing year. 197. d- Micronutrient treatments were more effective in increasing forage and dry yield early on each of the three growing years, mainly on the third and fourth cuts (June and JUly) rather than the late cuts of each year. Since alalfa is a perennial forage crop, itsplants in each growing year of the stand had its own physical and phy sLoLoq LcgL . ~tatus, which could respond differently to any of the applied cultural bratments. Extra more specific information about the effect of the applied micronutrient treatments under the various phosphorus levels on forage and dry alfalfa yield in each of the three growing years are presented as follows: Firstgrowin9 year 7- Forage yield a- At zero soil phosphorus application level, soil application of B+zn and foliat application of B prodoced the highest alfalfa forage yield which was 21.38 and 29.38 ton per fad, respectively. b- Soil application of B + Zn and foliar application of B + Fe + Zn produced the maximun fora~yield which was 30.68 and 38.43 ton per fad,respectively,at 200 kg super 19B . phosphate per fad. c- At 300 kg superphosphate per fad, the higheast forage yield was produced by soil application ofB+ Fe+Zn and foliar application of Fe+Zn, where the obtained forage yield waS 34.43 and 30.70 ton per fad,respectively. B- Dry yield a- In case of using zero phosphours level, the higest alfalfa dry yield was produced by applying B+Zn to the soil and B to the foliage where the dry yield was 5.26 and 7.39 ton per fad, respectively. 0- By applying 200 k~ superphosphate per fad, soil application of B+Zn and foliar application of B+ Fe+Zn produced the highest dry yield which was 7.4 and 9.46 ton per fad, respectively. c- At the highest level~f phosphor~s application B+F that was applie~ to soil and Fe+Zn that was sprayed to the folioge produced B.21 and 7.39 ton per fad,respectivlywhich were the top yields in these cases. 199. Second Slrowin:3year : 9- Forage yiel, d a- Soil application of B+Fe, and folior application of B+Fe+Zn produced the higest forage yield of 44.88 and 51.0 ton per fad,respectively under the zero soil phosphores application level. b- At the soil phosphorus application level of 200 kg superphosphate per f ad; soil application ofB + Zn and folior application of Fe+Zn produced the maximum forage yield of 61.52 and 69.33 ton per fad, respectively. c- However, at the extra higmer level of phosphorus (300kg superphosphate per fad), forage yield of 66.63 and 60.1 ton per fad was produced by applying either soil or foliar application of Fe+Zn. 10- Dry yield a- The highest dry yields of 9.64 and 10.51 ton. per fad were produced by 2pplying B+Zn to the soil or by spraying Fe to the fOliage, respectively under the zero soil phosphorus applicat~on level. b- By applying 200 kg superphosphate per 201. foliage, where the obtained dry yield was 39,65 and 42.05 ton per fad, respectively. c- At the extra higher soil phospherus application level (300kg superphosphate per fad). highest forage yield was produced by the same micronutrient treatments as in the secand growing year. Forage yield was 43.73 and 39.38 ton per fad for soil and foliar application of B+Fe and Fe+Zn,respectively. 12- Dry yield: a- Treatments that produced the higest forage yield were some what different than those produced the higest dry yield. Soil and foliar application of B + Fe produced the highest dry yield of 6.70 and 7.95 ton per fad,respsectively under the zero soil phosphorus application level. b- In case of applying 200 k~ sl,lperp\losphateper fad~ dry yield of 9.2 and 9.9 ton per fad were produced by ap~lYlng B+Zn to the soil and Pe+Zn to the foliage. c- However, at the extra higher soil application level of phosphorus, soil application of Fe+7.n ana foliar ap~lication of B+Fe+Zn produced the hiiqhe st;dry yield. 202. The behaviour of some ve~etative characters: The effect of the applied fertilizer treatments and seasonal variation on the number of stems pe rvsq meter, plant height, and leaf/stem ratio was studied. Date were recorded from the second cut of spring, summer, autumm and winter seasons of each of the three growing years. Results could be summarized as follows:- 13- Number of stem¥ /sq meter: APplying 200 kg superphosphate per fad produced an increase in the number of stems /sq meter as compared with the control (zero lvel). This trend was true for the three growing years with asignlficant difference in the last two years only. A- In the first growing year, the highest numbers of stems/sq meter were obtaimed by soil and folior application of B1 soil and folior application of Fe1 and either soil application of Zn or folior application of B+F at zero,200 and 300 kg superphosphate per fad, respectively. b- However, in the second growing year either soil application of B or foliar application of B+Fe1 soil application of B+Fe+Zn or foliar application of either Zn or B1 and soil application of either 203. ’ Zn or B+Zn and foliar application of B+Fe+Zn produced the highest numbers.of ~tems/sq meter at zero ,20Q,and 300kg superphosphate per fad,respectively. c- Meanwhile, the maximum number of stems/sq meter in the third year was obtained by soil application of Band foliar application of either B+Fe or B+Zn:’soil application of B+Zn+Fe or foliar application of B+Zn: soil application of Fe+Zn folior application of B+Fe at zerO,200,and 300 kg superphosphate per fad. d- Maximum number of stems/sq meter was produced in autumn, summer, and autumn in the first, second, and third growing years, respectively. e - Generally, foliar application of micronutrient treatments produced significantly higher number of stems/sq meter as compared with soil applic_ation in the first and third year, with no significant difference in the seQQnd growing year. 204. 14- Plant hei~ht: The effect of the applied fertilizer treatments on alfalfa plant height was fluctuating having no specific tremd in the three growing years. a- In the first growing year, tallest plants were produced by foliar application of Fe+Zn;soil and foliar application of Fe and B+Fe!Zn soil and foliar application ofB+Zn and Fe,resectively at 0,200,and 300 kg superphosphate per fad. b- In the second growing year, tallest alafalfa plants were produced by soil application of B+Fe foliar application of Fe+Zn at zerO soil phosphorus application level. And either soil or foliar application of Zn at 200 kg superphosphate, by soil application of Fe+Zn and foliar application of Fe at 300 kg superphosphate per fad. c- Soil and folior application of Zn and B+Fe+Zn,respectively; soil and foliar application of B+Zn and B+Fe,respectivelY;soil and foliar application of B+Fe and B+Fe+Zn produced the tallest alfalfa . plants under 0,200, and 300 kg superphosphate, resprectively in the third growing year. 205. d- Also, tallest plants were obtained in Summer season of the first and secJnd growing years, and spring of the third growing year. e- Generally, foliar application of micrountrients produced significantly taller plants as compared with soil application in the three growing years. 15- Leaf/stem ratio: a- Either soil or fOliar application of zn and B+~e+Zn prod~ced the higest leaf/stem ratios of alfalfa plants at zero and 200 kg superphosphate per fad1 resprectively in the first growing year. However, soi1app Li.catLon of Fe+Zn and folia r applieation of B+Zn produced the highest leaf/ stem ratios at 300 kg superphosphate per fad. b- During the second growing year, soil and foliar application of B at zero and 300 kg superphosphate per fad produced the highest leaf/stem ratio~ of alfalfa. However at 200 kg superphosphate per fad. soil application of B or Zn and foliar application of B produced the highest leaf/stem ratio. 206. c_ In the third year, soil application ofZn,8+Fe+zn, and Fe+Zn; and foliar application of 8+Fe±Zn,Fe, and Zn produced the highest leaf/stem ratio of alfalfa at 0,200,and 300 kg superphosphate per fad, respectively. d- More tendency for high leaf/stem ratio was obtained in spring of the first and third growing years, and winter of the second growing year. e- Methods of micronutrients application did not produce a significant difference in leaf/stem ratio of the last two growing years, and a significant difference in the first growing year in favour of foliar application. 16- Chemical anaLysis: The effec~of the applied fertilizer treatments and seasonal variations the chemical analysis of alfalfa leves and stems were investigated in the second growing year. Randomly samples of 100 grams of leaves and stems were 207. taken from each individUal sucessive cut of spring, summer autumn, and winter seasons. Fresh samples of all cuts of each season were oven dried, ground and mixed together thoroughly to form a representative composite dry samples of leaves and composite dry samples of leaves and stems for each season to be used for chemic?l analysis. Results will be limited for leaves tather than stems for a var iety of .reasons which were listed in the text. Results are summarized as follows:- Crud protein content: a- Applying 200 kg superphosphate per fad produced more crud protein content of alfalfa leaves as compared with the control. No further increase in crud protein content was obtained at the higher phosphorus level. b- Crude protein contents were increased from spring to summer season, then slightly decreased in autumn, then winter at all of the applied phosphorus levels. c- Methods of micrenutrients application did not affect crud protein cOntents in spring and winter. However, higher crud protein cOntents were obtained by soil application in autumn , and foliar application in summer. 208. d- The highest crud protein contents of leaves in spring, summer, autumn, and winter were produced by zn (22.8%), Fe+Zn (22.5%), B+Zn (25.0%), and B+Fe~Zn (19.37), respectively. Zn participated in all of these tratments. E- Generally, foliar application of each of the applied micronutrient teatments was more productive as far as crud protein content is concerned than soil application excepet for B. B- Phosphorus content: A- Applying 200 or 300 kg superphosphate per fad produced higher crud protein. content in leaves as compared with the control. b- Phosphorus accmulation was high in spring and summer (0.22%), low in autumn (0.20%), and lower in winter (0.18%). c- Any of the applied micrOlilutrients·:tJ::eaments either to soil or foliage increasedaccumulation as compared with the control. d- Phospharus contents were neither affected by the various micronutrients treatments nor the application methods in all seaons. 209. c- Ferrous content: a- Ferrous contents of alfalfa leaves dec.reased as the phosphorus application levels increased. Applying 200 and 300 kg superphosphate per fad reduced Fe content of alfalfa leaves as compared to the control. b- There was a .cont in uou s drastic reduction in Fe content of alfalfa leaves from spring to summer, autumn, then winter. d- Foliar application of micrountrients increased Fe contents in soring and autumn seasons as compared with soil application. However, .soil application of micronutrients increased Fe contents of leaves in summer and winter seasons as compared with foliar ap~lication. E- Highest Fe contents of alfalfa le~, ves were obtained by foliar application of Fe in spring (560 ppm) and summer (240 ppm), B+Fe+Zn in autumm (583 ppM), and B+Fe in winter (517 ppm). However, soil application of the following treatments produced the highest Feco~tentg~Fe-in 210. spring (363 ppm), B+Fe in summer (3fl7 ppm), B+Fe+ Zn in autumn (213 ppm) and winter (630 ppm). F- Averaged overall the four seasons, foliar application of B+Fe+Zn , B+Fe,Fe+Zn,Fe, and Zn increased Fe contents of alfalfa leaves by 18,11, 10,8, and 3%,respectively as compared with soil application method. D- Zinp content : a- Zin~ contents in alfalfa leaves was not affected by the various soil phosphorus application levels.Averaged overall of the applied micrountrients application,methods of application did not affect Zn contents of alfalfa leaves in autumn and winter season b- However foliar application of micrountients increased Zn contents of alfalfa leaves by 36% as compared with soil application in spring season. 3nwhile, soil application of micronutrients produced more Zn contents in alfalfa leaves by 45% as compared with foliar application. c- Any of the applied micrountrient treatments whether as soil or foliar application :”caused an increase in Zn contents as compared with the control. 211. d- The highest contents of Zn in alfalfa leaves were abtained by foliar application of Fe+Zn in spring (80 ppm), B+Zn in summer (47 ppm), B+Fe+Zn in autumn (46 ppm), Zn or Fe In winter (47 or 48 ppm) Howerer, soil application of Zn in spring (62 ppm), B in summer (63 ppm), B+Zn+Fe in autumn (46 ppm), and B+Zn in winter (63 ppm) produced the highest Zn contents in alfalfa leaves. E- On the average, methods of application of each micrountrient treatments did not show a marked. effect on Zn contents of alfalfa leaves. |