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Abstract SUMMARY - The present investigation was carried out in an attempt to give some information about the growth of Azolla caroliniana under di”fferent levels of some growth regulators such as GA3’ IAA, MH and B-9. To approach this aim, two laboratory experiments were carried out under semi-controlled conditions which include 1- Response of Azolla caroliniana plants to GA3 and IAA. 2- The interaction effect of GA3. IAA. MH,B-9 and ’theil”i”nteraction on Azolla carol iniene plant. This investigation was carried out in nutrition lab., Institut. Oceano. Fisher. Reserch. NIOF. Egypt. during 1986.1987 including two experiments Experiment I _ IAA :Applied at rates 5.10.20.30.40 and 50 p.p.m. _ GA3 :Applied at rates 10.20.40.60.80 and 100 p.p.m. Experiment II : IAA :Applied at rates 1.2 and 5 p.p.m. _ GA3 :Applied at rates 0.1.0.5 and 1 p.p.m. B-9 :Applied at rates 20.40 and 100 p.p.m. _ MH :Applied at rates 1.10 and 30 p.p.ro. And their interactions. 137 The following data were recorded. a) Fresh weight/vessel. b) Numberof fresh fronds/gram. c) The numberof fronds/vessel. cl.>:·Fresh weight/frond. - . e) Dry weight/vessel. f) Dry weight/frond. g) Growth percentage compared with starting sample. h) Determination of Macro-nutrients content. i.e. N,P, K and Micro-nutrients, i.e. Mn. Zn. Cu.~ The results obtaind could be summarized as’ follows :- A) Experiment I : a) Growth behaviour 1) Both tested growth promoters. GA3 and IAA, seemed to minimized the development of Azolla fresh weight and dry as compared with the un.t.r-e ated control plants. and GA3 seemed to have a saperior effect than IAA in this respect. b) Chemical composition 1) The untrea.ted plants possessed the highest nitrogen concentration as compared with other treatments as well as starting sample. The various levels of GA3 depressed greatly the nitrogen concentration while the levels of IAl stimulated it: . ’ 138 2) The levels of GAs seemed to depress the atmospheric nitrogen fixation. The treatmentsof IAA seemed to have lower depressive effect on the nitrogen fixation activity than GA3 treatments. .•• > 3) Both GAs and IAA levels affected the· accumu la-r ’ tion of phosphorus in Azolla plants. Both GA3 and IAA reduced the concentration and total phosphorus amounts in Azolia plants; IAA treated plants possessed higher concentration of phosphorus than the starting sample. 4) The treatments of GAs or IAA declined the absorption ofpotassium by Azolla plants. GA3 levels showed higher depressive effect than IAA in this respect. 5) The Azol1 d plants possessed high amounts of Mn followed by Zn while eu was the lowest. The total amounts of these micro-elements showed a continous decrease with the application of GA3. while no clear trend was obseved in the case of IAA except a decrease in total amounts of the three nutrients. - 139 B) Experiment II : a) Growth behaviour 1) The presence of the tested growth regulators. in nutrient media of Azolla plant reduced the production of fesh weight biomass per vessel except Kylar as such,anti-GA ~”which stimulated ”the production of fresh· weight per vessel over the control. All treated plants produced higher fresh weight than the starting sample. 2) The increase of GA3 levels decreased gradually the fresh weight biomass under fixed Kylar level. However. IAA stimulated greatly the depressive effect of GA3 on fresh weight biomass. 3) Kylar seemed to minimize the depressive effect of 11\1\ at the moderate and high level. MH antagonist the depressive effect of IAA at the lowest level. 4) The GA3 single and IAA levels have a relatively retarding effect on dry weight production of Azolla bIomass per vesse I. Kylar treatments adverse the depressive effect of GA3 on dry weight biomass. The using of MH seemed to have more or less than an adverse action on the depressive effect of GA3 on dry weight per ves- ~ sel, while IAA stimulated the depressive effect of GA3 on dry weight per vessel. Kylar adversed the inhibitory effect of IAA as it was showed by GA3-Kylar interaction. MH seemed to adverse the inhibitory effect of IAA. 140 5) ·Generally all the tested growth SUbstances. increased the fragmentation of AzalIa biomass over the control treatments. b) Chemical composition : 1)”The different treatments with single growth substances seemed to have higher nitrogen concentration compared with starting sample while most treated plants possessed less nitrogen concentration than the control. Kylar seemed to a stimulatory effect on nitrogen fixation. 2) Kylar application seemed to regulate and diverse the retarding effect of GAa on nitrogen fixation. MH treatment diversed the inhibitory effect of GAa on nitrogen fixation. Kylar diversed the depressive effect of IAA on N fixation. 3) The growth substances affected the accumulation and absorption of phospgorus by AzolJa plants. 4) GAa and IAA have a retarding effect on phosphorus absorption. Kylar seemed to have a stimulatory effect on the absorption rate of phosphorus. this stimulation was extended as reversable action over the depressive effect of either IAA or GA3. 5) GAa• lAA and MH seemed to retard the accumulation of K while. Kylar stimulated ,uch accumulation. PotasDum absorption rate trend seemed to be the same of 141 ----’----- ---- --- phosphorus and nitrogen. IAA and GA3 retarded the absorption rate of potasium while Kylar and MH stimulated such absorption. 6) The absorption of Manganese was continuous under the different treatments as compared with starting sample. ~~ 7) kylar decreased the concentration of Zinc. GA3 stimulated Zinc concentration but such increment decreased gradually with increasing GA3 level. With regard to the effect of MH on the effect of GA3 or IAA. most of the used rates declined Zinc concentration in Azolla plant tissues. 142 ~~-- -~.’-’ |