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العنوان
Adaptation studies on increasing salt tolerance of mung bean /
المؤلف
Ahmed, Y. A. M.
هيئة الاعداد
باحث / Y.A.M.Ahmed
مشرف / M.R.Gabal
مناقش / A.S.Shafshak
مناقش / H.S.Khafaga
الموضوع
Beans.
تاريخ النشر
2003.
عدد الصفحات
99 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
البساتين
تاريخ الإجازة
1/1/2003
مكان الإجازة
جامعة بنها - كلية الزراعة - بساتين
الفهرس
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Abstract

The present work was carried out during 2001; 2002 and 2003 years in the Tissue culture Laboratory of Horticulture Research Institute, Giza, Governorate to investigate the in vitro propagation of common fig and guava through tissue culture technique using shoot tip and nodal cutting explants from two cultivars of each fruit species i.e. (Kadota & Black Mission) and (El-Sabaheya & Banaty) cultivars for former and later species, respectively. The conducted experiments during each stage were as follows:
V.I. Establishment stage:
V.I.1. Effect of antioxidant agents on browning :
In this regard specific and interaction effect of: a- Four antioxidant treatments (adding PVP to culture medium at 1000 mg/L and soaking for 2 hrs in ascorbic acid + citric acid solution at 100 & 150 mg/L from each either alone or plus adding PVP to media, beside soaking in distilled water as control) ; b- Culture media (MS; WPM and B5 each at its full strength basal nutrients) and C- their possible combinations (3 media X 4 antioxidant treatments) were investigated. Hence, an experiment (factorial) was devoted for each explant type excised from every cultivar (8 factorial experiments). Browning % of cultured explants was recorded after 4 weeks from culturing and incubation.
V.I.2. Survival % and growth of cultured explants as influenced by culture medium and its strength:
The survival % of cultured explants (each separately) and their growth (explant height & number of leaflets per each) in response to specific effect of culture media (MS; WPM; B5) and strength/concentration of their basal nutrient elements (full, one half and one fourth strength), as well as their possible combinations (3 culture media x 3 strength) were investigated after four weeks from culture and incubation.
V.H. Shoot multiplication stage:
In this concern specific effect of culture media (MS; WPM; B5) each at its full strength basic salts) and cytokinins supplemented to culture medium (9 treatments of 3 cytokinins kinds i.e. 2iP; BA; Kinetin each added solely at one of 2, 4 or 6 mg/L, beside cytokinins omission medium as control), as well as interaction effect of their possible combinations (3 culture media x 10 cytokinins treatments) were investigated after four weeks through each subculture (3 ones) during this stage (shoot multiplication). Cultured shootlets of each subculture were incubated for four weeks at 25 ± 2 C*; RH of 70-80 % and exposed to the daily light/dark cycle of 16/8 hrs., respectively . At the end of each subculture (4 weeks from culturing) number of proliferated shootlets per each explant; average length of each shootlet and number of developed leaflets per each were recorded.