الفهرس 
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Abstract
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Summary
The present study consists of four parts. The first part of
experiments was conducted at the Experimental Farm of Plant
Res. Dept, Nucl. Res. Center, Inshas during three successive
growing seasons (2001 /2002,2002/2003 and 2003/2004) to
evaluate the selected lupin mutants for earliness and yield and its
components characters in M3, M4 and M5 generations.
The second part was conducted at the laboratories of plant
Res. Dept .,Nud . Res. center, Inshas during three generations to
evaluate the induced mutants for seed quality traits
The third one was conducted at the unite of stored product
insects, Radiobiology Dept, Nucl. Res. Center, Inshas, during
three generations M3, M4 and M5 to evaluate the induced
mutants especially ones with low alkaloid content to infestation
by two insects i.e. Callosobruchus chinensis and Heliothis
armigera.
The fourth part was to study the correlation between yield and
its components, alkaloid and Protein content and the
susceptibility of these mutants to infestation by Callosobruchus
chinensis and Heliothis armigera.
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The induced mutants were selected from irradiated M2
populations with gamma ray doses 0, 300 , 400 and 500 Gy and
the treated M2 populations with EMS concentrations 0 , 0.1 , 0.2
and OJ
Some promising mutant lines (23 mutants) as well as the two
local cultivars (Giza 1 and Giza2) were evaluated in M3 , M4
and M5 generations. In each generation, complete randomized
block design with three replications was performed to detect the
performance of these mutant lines.
- The results of this study could be summarized as
follows:-
1)The analysis of variance for different studied characters
showed highly significant differences among different lupin
genotypes in the three generations (M3, M4 and M5), except
for number of seeds / plant trait in M3 generation and weight
of 100 - seed weight trait in M4 generation which indicated
insignificant differences among lupin genotypes.
2) For flowering date, the mean performance ofM3 revealed that
earliness was observed for mutant lines 5 , 11 , 17 , 19 , 21
and 22 as compared with the two local cultivars Giza 1 , and
Giza 2 with maximum value of 19 days earlier than Giza2.
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For M4 generation, earliness was also noticed for mutant
lines 5 , 11 , 17,21 and 22 as compared with the two local
cultivars. However, for M5 generation, earliness was only
obtained for mutant lines 5 and 21 only as compared with the
two local cultivars. Paradoxically, most mutant lines showed
lateness as compared with the two local cultivars in the three
generations M3, M4 and M5 with maximum value of 9 days
delayed than that of Giza2.
3) For number of pods/plant, the mean performance of the three
generations (M3 ,M4 and M5), indicated, that highly
significant increase for this trait was obtained for mutant lines
8 , 15 and 19 as compared with the two local cultivars,
However, most mutant lines showed marked increase for this
trait as compared with Gizal in the three generations (M3,M4
and M5).
4) For number of seeds/plant, the mean performance for the three
generations (M3, M4 and M5) showed that a considerable
increase for this trait was noticed for different mutant lines as
compared with Giza!. However, marked increase for this trait
was only obtained for mutant lines 8 and 15 as compared with
Giza2 in the three generations.
5) For 100- seed weight, most mutant lines showed significant
increase for seed index as compared with Giza 1 in M3 and
M5 generations. However for M4 generation, a considerable
increase for seed index was only observed for mutant lines
6, 12, 15 and 18 as compared with Giza 1 whereas, in
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comparison with Giza2, marked increase for this trait was
obtained for 6,7,9, [2,14,15,16,18,20 and 21 in M3.
However, markable increase for this trait was only obtained
for Mut6 ant Mutl2 in M4 generation. But significant increase
for seed index was only obtained for mutant lines 12, 17 and
21 in M5 generation.
6) For seed yield / plant, the mean performance showed that a
considerable increase fur seed yield / plant was obtained for
mutant lines 1,4,7,8,15 and 19 as compared with the two
local cultivars in the three generations M3, M4 and M5.
7) For alkaloid content the mean performance showed that
highly significant decrease for alkaloid content was obtained
for mutant lines 20 and 23 as compared with the two local
cultivars in the three generations. However, insignificant
reduction was noticed for most mutants as compared with
Giza 2. also, insignificant decrease was noticed for mutant
lines 1 , 5 and 11 as compared with Giza 1.
8) For protein content, the mean performance showed that except
for mutant lines 1, 5 and 11, all mutant lines and Giza2
showed marked increase for protein content as compared with
Gizal in the three generations M3 ,M4 and M5. However, the
increase in protein content did not reach the level of
significance for most mutant lines as compared with Giza 2 in
M3 and M5 generations, but, a considerable increase in seed
protein content was obtained for mutant lines 4,8, 10, 14, 17,
20 and 23 as compared with Giza2 in M4 generation.
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9) For infestation by C. chinenses, the mean performance
showed that,except of mutants 1, 5 and 11,most mutant lines
showed significant increase for infestation percentage as
compared with the two local cultivars Gizal and Giza2 in the
three generations (M3, M4 and M5) suggesting that Mud,
MutS and Mut 11 were less infestation, however, mutant lines
6, 9, 12, 15 and 21 were moderate infestated, while most
mutant lines were susceptible for infestation.
10) For infestation by H.armigera, the mean performance
showed that the lowest infestation by H. armigera was
obtained for Mud, Mut5, Mud 1 and local variety Gizal in
M3 and M5 generations as compared with other mutants
which showed significant infestation for this insect. However
in M4 generation, no infestation was only obtained for Gizal
and Mut5 as compared with other mutants which showed
marked infestation.
11) For total infestation by the two insects, (C. chinenses and
H. armiger), the meanperformanceshowedthat lowinfestationby
the two stored products insects for Mud, Gizal and Mutl l as
compared with other mutant lines and Giza2 that showed
significant infestation by the two insects in M3 and M5
generations.However,in M4generation,the low infestationwas
obtained for Mut4 and Mutl 0 as compared with other mutant
lines and the two local cultivarsGizal and Giza2
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12) For loss percentage, except of mutant lines 1,5 and 11,
different mutants showed higher loss percentage than that of the
local cultivars Giza 1 and Giza2 in the two generations M4 and
MS.
13) For correlation studies, in the three generations, alkaloid
content showed high negative correlation with protein content,
infestation by C. chinensis, H armigera and total infestation by
the two insects. Protein content showed high positive correlation
with infestation by C. chinensis, H armigera and total infestation
with the two insects, No. of pods/plant and lOO-seedweight. Also
high positive correlation was observed between yield/plant and
number of pods/plant.