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Abstract Non-surgical embryo recovery and transfer techniques have a great economic potential for the rapid improvement of c~ttle and II give a better chance for genetic selection to incre~se production • The present study aimed for the applicatioD of Don-surgical eabryo transfer technique in 4 breeds of german cattle with trails to use different filter systeas to define the most sui! o table one. Horeover, responsivenes of donor aniaals for superovulation with PHSG (3000 lU) and FSH (46 mg) horNones I1 1 were estimated. Synchronizations of oestrus with Estrumate in recipient animals were performed. The influences of season, embryo stage and embryo quality UpOD pregnancy rates after the used non-surgical embryo transfer were observed. The number of saccessful non-surgical flushing was 55 out of 60 prepared cows. Tbe donor animals used in the present work were 19 Scb.arzbnn~, ’8 lotbun~, lQ Fleckvieb and two Cbarolal. cows. The Dsed flushing mediua was nulbecco’s phosphate buffered saline (PBS) with 1% beat inactivated fetal calf serum (FeS). Tbe vsed ! flusbing catheter was Reu.ta’t/Ai.cb type and the transfer Ij ! Ii cannula was of Baunover ao.el. The used filter eystems in the preseDt v~rk have a variable tecbniques • The siphoning tecbnique t filters vere AI••r.’orf 81d Bialtub ao••I. while, tbe .ieveing tecbnlque encluded EaC.. aDd overllowfng tecbnique was Vel 101The percentage of animals responded to PHS~ ments ~ere 90% and 91.33% respectively. The rates embryos which recovered from Schwarzbunt. Rotbunt. Charolais were 80.62 %. 63.55 X. 38.62 % and 33.33% Higbly significant difference (p < 0.01) was noted percentages. The rates of recipient aniaals responded to injection each one 500 ug) were 86.60%. 79.26 %. 75.0% and 61.54% in Schvarzbumt • totb.mt • Fleckyieh and Charolais cows reapectively. Si~nificant difference (p < 0.05) was noted between these results. The total pregnancy rate in tbe present work was 61.62%. HoweYer. it varied according to the season of the year. The obseryed pregnancy rates were 62.04%. 60.89%. 59.46% and 64.87% in Winter. Spring. Summer and Fall respectively • The pregnancy rates appeared to be very low wben embryos were tranaferred ~o recipient aniaals in October (54.55 X). April (55.39 %) • July (57.14 %) and January (57.78~). However. these rates becaae 72.41%~ 11.05% and 69.81% In December. Noyember and Narch respectiYely. Tbe season and month of the year have no significant effect upon sucb results. |