الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
This study started with a good try to find success and actual protocol for regenerated complete and fertile plants form Egyptian cumin cells in vitro, so it used hypocotyls as a source for these cells and produced many calli through different four protocols (8 nutrient media and illumination regime) and regenerated many fertile plants. The first protocol (B5 medium containing 0.215 mg/l kinetin under darkness) was the best, so it is used for the next experiments of the present study. The study recorded for the first time success in in vitro isolation and embryo cultures from Egyptian cumin through the experiments which have been conducted via three protocols with regenerated complete and fertile plants from it (as from direct or indirect regeneration), so this will open door to use the embryos in genetic engineering or in vitro selection to produce plants with desired characters e.g. stress-tolerant plants.
Establishment of regenerable highly embroygenic cell suspension cultures was performed from embryogenic cumin calli, the growth dynamic of suspension cells was measured through one year. The cells were cultured on solid media and produced calli and regenerated plants (with ratio 820 plants/100ml cell suspension). The chromosomes numbers of suspension cells, their regenerated plants and regenerated plants from hypocotyl calli, there were varied with its numbers (between 7-28 chromosome), however, most of them contains the normal chromosome number of cumin (14 chromosome). The study made actual protocol for selection of resistant cumin calli to fusaric acid (150 µM, the most important of Fusarium fungi poisons) and regenerated plants from putative resistant calli.