الفهرس | Only 14 pages are availabe for public view |
Abstract The aim of this work was to study the growth response of S. persica in vitro via micropropagation and consequently the production of identical true type plantlets using shoot tip and nodal cutting explants. Micropropagation of S. persica was achieved by using shoot tips and nodal cuttings through the starting and rooting stages. The effect of growth regulators was investigated. The best medium for shoot tips and nodal cutting was MS basal medium supplemented with 2.0 mg/l kinetin and 0.1 mg/I IAA. This balance stimulated 66.6% of shoot tips to form new shoots with 88.9% survival and 11.1 % of them formed callus. The same balance stimulated 66.6 % of nodal cuttings to form new shoots with 83.3% survival and about 16.7 % of them could form callus. Preliminary phytochemical screening identifies the presence of carbohydrates and/or glycosides, free flavonoids, sterols and/or triterpenes, alkaloids as well as tannins more strongly with shoot samples propagated from tissue culture technique indicating their occurrence in such samples with higher amounts. The UV and the visible spectra were recorded by the spectrophotometer for the tested extracts prepared from both the wild and tissue culture propagated plant of S. persica. The UV/visible spectra of the ethanol 95% extracts of both the wild and tissue culture propagated plant were similar. The intensities of the absorption bands were higher with those extracts prepared from the plant propagated by tissue culture technique and extracted with chloroform, ethanol 70% and absolute. |