الفهرس 
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Abstract
Summary:-Yeast of genus Malassezia is a part of the human skin and worm blooded animal’s flora. Malassezia species lipophilic unipolar yeast recognized as commensal of the skin that may be pathogenic under certain conditions. This lipophilic yeast associated with various human and animal diseases.
A total of (90) samples have been collected from both diseased human, diseased and apparently healthy animals. Human samples were collected from cases of tinea versicolor and seborrheic dermatitis as skin scrapings while animal samples were collected from ear and skin of diseased and apparently healthy animals as ear swab and skin scrapings, samples were cultured on mycobiotic agar, PDA with Cloramphenicol + Cyclohexamide with sterile olive oil covered the inoculated site and SDA with Cloramphenicol + Cyclohexamide with and without sterile olive oil which revealed 29 Malassezia species.
As regard to human samples, (14) Malassezia isolates were obtained from (20) samples which identified into (3) Malassezia species namely M. furfur 9 (64.28%), M. sympodialis 4 (28.57%) and M. globosa 1(7.1%).
Concerning to 70 samples collected from apparently healthy and diseased animals, 15 Malassezia isolates were obtained from them 13 M. pachydermatis with frequency of (86.67%) and 2 M. furfur with (13.3%).
The isolates were identified into Malassezia species by using the phenotypic method which including morphological characteristics (macroscopicmorphology and microscopicmorphology) and physiological characteristics of the isolated Malassezia species. Macromorphological characters as rate of growth, size, shape, colour of colonies and consistency were reported after subculture on Dixon’s medium, potato dextrose agar and Sabouraud Dextrose agar. Microscopy of isolates was done by preparing film of the colonies then stained by Gram stain to demonstrate and examine size and shape of the yeast. The physiological characteristics were done by growth on different temperature on Dixon’s medium (32oC, 37oC and 41oC), catalase test, growth on tryptophan medium and Tweens assimilation test.
Malassezia isolates were subjected for antifungal sensitivity test using Itraconazole, Ketoconazole, Fluconazole, Clotrimazole, Nystatin and Amphotericin. Antifungal sensitivity test for M. furfur, M. sympodialis, M. globosa and M. pachydermatis, which obtained from this work revealed the susceptibility degree to Itraconazole and Ketoconazole followed by Fluconazole in case of M. furfur, M. sympodialis and M. globosa. Nystatin showed high effect in case of M. pachydermatis followed by Ketoconazole, Fluconazole and Itraconazole.