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Abstract
Our study was based on constructing 2 experiments:
1-Constructing a metabolic profile test:
It was done_on 20 highly lactating,multipareous dairy cows at 2weeks(- 1 4day) prer calving,lweek(-l-7 days) and 3 weeks(+2lday) post-calving to detect the metabolic
changes that occur in the blood comopOnents in dairy coe
ws during this crirical period of the productive cycle of dairy cow at which it changed from non-lactating,pregnant to lactating ,non-pregnant state.
Two blood samples from each cow were collected and treated As the following:
1 -The first one contained anticoagulant (EDTA)which used for collection of unclotted blood for haematological analysis for estimating haemoglobin (gm/lOOml), PCV (%), RBCS coun (X106/lOOml), WBCS count(X103/lOOml) and differential leucocytic count.
2-The second tube without anticoagulant in which the blood is left to coagulate at room temperature till the clot retracted , then centrifuged at 3000 r.p.m for 10 minutes to remove any residual blood cells then the clean non haemolyzed sera were transferred carefully to clean and sterile vials with stopper. These sera were stored at20C°.
These serum samples were used for estimating: Calcium , inorganic phosphorus ,Ca/Ph ratio, magnesium, sodium , potassium , total protein, albumin, globulin ( by subtracting the albumin from the total protein values ) ,A!G ratio, urea , glucose, cholesterol ,triglycerides and total bilirubin.
Our results revealed that:
1-Biochemical analysis:
1-Serum calcium and phosphorus was significantly decreased at the first week after calving than its level at the last 2 weeks before calving and the third week after calving.
2- Serum magnesium level was lower but not significantly decreased in dry cows than in lactating cows.
3- Serum sodium level was significantly increased in the last 2 weeks prepartum than its level at the first and third week after calving,while serum potassium level was not significantly changed either before or after calving.]
70
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Our study was based on constructing 2 experiments:
1-Constructing a metabolic profile test:
It was done_on 20 highly lactating,multipareous dairy cows at 2weeks(- 1 4day) prer calving,lweek(-l-7 days) and 3 weeks(+2lday) post-calving to detect the metabolic
changes that occur in the blood comopOnents in dairy coe
ws during this crirical period of the productive cycle of dairy cow at which it changed from non-lactating,pregnant to lactating ,non-pregnant state.
Two blood samples from each cow were collected and treated As the following:
1 -The first one contained anticoagulant (EDTA)which used for collection of unclotted blood for haematological analysis for estimating haemoglobin (gm/lOOml), PCV (%), RBCS coun (X106/lOOml), WBCS count(X103/lOOml) and differential leucocytic count.
2-The second tube without anticoagulant in which the blood is left to coagulate at room temperature till the clot retracted , then centrifuged at 3000 r.p.m for 10 minutes to remove any residual blood cells then the clean non haemolyzed sera were transferred carefully to clean and sterile vials with stopper. These sera were stored at20C°.
These serum samples were used for estimating: Calcium , inorganic phosphorus ,Ca/Ph ratio, magnesium, sodium , potassium , total protein, albumin, globulin ( by subtracting the albumin from the total protein values ) ,A!G ratio, urea , glucose, cholesterol ,triglycerides and total bilirubin.
Our results revealed that:
1-Biochemical analysis:
1-Serum calcium and phosphorus was significantly decreased at the first week after calving than its level at the last 2 weeks before calving and the third week after calving.
2- Serum magnesium level was lower but not significantly decreased in dry cows than in lactating cows.
3- Serum sodium level was significantly increased in the last 2 weeks prepartum than its level at the first and third week after calving,while serum potassium level was not significantly changed either before or after calving.]
70
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