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العنوان
studies on in vitro propagation of aswan date palm cultivars and their germplasm preservation /
المؤلف
abu el-fadl, reda el-sayed el-sayed.
هيئة الاعداد
باحث / Reda El-Sayed El-Sayed Abu El-Fadl
مشرف / Mahmoud Imam Nasr
مشرف / Ibrahim Abd El- Maksoud Ibrahim
مشرف / Mahdia Farid Gabr
الموضوع
Date palm.
تاريخ النشر
2008.
عدد الصفحات
p298. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
Biochemistry, Genetics and Molecular Biology (miscellaneous)
تاريخ الإجازة
1/1/2008
مكان الإجازة
جامعة المنوفية - كلية الزراعة - Plant Biotechnology
الفهرس
Only 14 pages are availabe for public view

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Abstract

This study was conducted from ٢٠٠٢ to ٢٠٠٨ on four dry date palm cultivars i.e., Gondeila, Bartamuda, Sakkoty, Malakaby .The study comprised two parts : Part I : - In vitro propagation of the four cultivars via somatic embryogenesis : - Study the genetic stability between the mother plant and the produced plants from micropropagation using RAPD analysis: - Gondeila cv. gave the highest significant survival percentage, followed by Sakkoty and Bartamuda, cvs. while leaf primordia gave the highest significant survival percentage. Multi- step treatment using ٣ % NaOCl followed by ٠,١ mg/L HgCl٢ and followed by ٢٪ NaOCl gave the highest significant survival percentage. - January was the most suitable time for explant collection, which gave the lowest browning percentage, .- Culture medium containing ١٠٠ mg/L ٢،٤-D + ٣ mg/L ٢iP was most effective on swelling percentage. - Bartamuda cv. achieved the highest value of callus initiation percentage whereas Malakaby cv. gave the lowest percentage. Subculture ٣ achieved the highest browning degree. Culture medium containing ١٠ mg/L ٢،٤-D + ٣ mg/L ٢iP gave highest degree of browning. - Shoot tip explant exhibited the highest embryogenic callus formation. Culture medium containing ٠,١ mg/L NAA gave the highest percentage of callus differentiation .