![]() | Only 14 pages are availabe for public view |
Abstract This study was conducted from ٢٠٠٢ to ٢٠٠٨ on four dry date palm cultivars i.e., Gondeila, Bartamuda, Sakkoty, Malakaby .The study comprised two parts : Part I : - In vitro propagation of the four cultivars via somatic embryogenesis : - Study the genetic stability between the mother plant and the produced plants from micropropagation using RAPD analysis: - Gondeila cv. gave the highest significant survival percentage, followed by Sakkoty and Bartamuda, cvs. while leaf primordia gave the highest significant survival percentage. Multi- step treatment using ٣ % NaOCl followed by ٠,١ mg/L HgCl٢ and followed by ٢٪ NaOCl gave the highest significant survival percentage. - January was the most suitable time for explant collection, which gave the lowest browning percentage, .- Culture medium containing ١٠٠ mg/L ٢،٤-D + ٣ mg/L ٢iP was most effective on swelling percentage. - Bartamuda cv. achieved the highest value of callus initiation percentage whereas Malakaby cv. gave the lowest percentage. Subculture ٣ achieved the highest browning degree. Culture medium containing ١٠ mg/L ٢،٤-D + ٣ mg/L ٢iP gave highest degree of browning. - Shoot tip explant exhibited the highest embryogenic callus formation. Culture medium containing ٠,١ mg/L NAA gave the highest percentage of callus differentiation . |