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Abstract
Babesiosis one of the most important diseases that limits the
production and improvement of animal production in tropical and
subtropical regions. cysteine proteases is an important enzyme for
the life cycle and pathogenisis of many parasites. Babesia microti
was used as a model for babesia species in this study. Two different
genes of cysteine proteases were found in the genome of babesia
microti, one of them is a single copy (CP1) and the other is a multiple
copies (CP2). The whole sequence of cysteine protease genes of
babesia microti were isolated. Activity of cysteine proteases was
determined on SDS-PAGE copolymerized with 0.1 % gelatin by using
,
of rabbit anti synthetic peptide. Finally, the importance of cysteine
proteases for the life cycle of some babesia species (babesia bovis
and babesia equi) were determined by using of many cysteine
proteases inhibitors like E64D, Calpain and Epibestatine invitro.