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Abstract
Acute myeloid leukemia is a haematologic malignant disorder
characterized by uncontrolled proliferation of myeloid progenitors. AML
account for 15-20 percent of acute leukemias in children.
Cytogenetic analysis of AML patients to identify different
chromosomal abnormalities, specially t(8;21) translocation which is the
most common, is very important as a prognostic clue and for treatment
planning because each abnormality has different prognosis and outcome
of treatment.
The aim of this study was to demonstrate the percentage of
different chromosomal abnormalities among AML children using
conventional karyotyping by G-banding technique and to demonstrate
t(8;21) translocation among those children using fluorescence in situ
hybridization (FISH) technique.
Twenty (20) AML children were included in the study (I Imale and
9 female) in the period between April 2000 and march 2002, in the
Oncology unit, Pediatric Department of Zagazig University Hospital. All
the cases were between 2 months - 13 years old with peak age group
between 2-7 years (55%) (Table 1).
The patients were subjected to full history taking, complete clinical
examination, complete blood count, bone marrow examination.
imrnunopheno-typing, liver function tests and cytogenetic analysis w-hich
include. t(8;21) translocation by G-banding technique and ”FISH”
technique.
]20 SummaryandCone/itsion
Our results showed that there was about 9% of our AML patients
(successful cases) being positive for t(8;21) which is compatible with
other reported data.
We found significant correlation between positive cases for t(8;21)
and median survival (Table 14). Since there was highly significant
correlation between median survival and the fate and the response to
treatment, so there is significant relation between positive cases and the
fate and the response to treatment.