الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Totally 117 different marine actinomycetes were isolated from 12 different marine samples collected from Ras Sedr, Ain Sokhna and Hurghada. An isolate coded C4 was selected for further study as it exhibited high proteolytic activity and was identified as Streptomyces albidoflavus based on morphological physiological, biochemical and chemo- taxonomical approaches according to ISP. The culture conditions were standardized for the growth and protease production in Streptomyces albidoflavus C4. . This isolate exhibited optimum enzyme production with culture medium pH of 10, inoculum size of 10% from stock spore suspension having CFU value of 9.6x108 cell/ml and with a culture volume of 50ml in 250ml Erlenmeyer flask .Different carbon sources in addition to various locally available agricultural residues have been screened for alkaline protease production. Glucose and sodium hydroxide-treated bagasse pith proved to be efficient carbon source for alkaline protease production, they showed the highest enzyme activity (110.17 and 121.46U/ml, respectively) and productivity (27.89 and 20.66U/mg protein, respectively). Casein in presence of yeast extract was considered as the best nitrogen source. The alkaline protease enzyme exhibited an optimum activity at pH 9 and was stable over a pH range from 6 to 11. At pH 12, about 75% enzyme loss had been detected and at pH 13 only 14% of the enzyme activity remained