الفهرس 
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Abstract
Typhoid fever was diagnosed by blood culture in 13 patients (9 males and 4 females) out
of 300 blood samples collected from suspected patients. Thirteen Salmonella typhi
isolates and three reference strains (090 I, H901, and Ty2) were subjected to molecular
characterization. The quality and quantity of Salmonella typhi DNA were checked by
amplifying ribosomal DNA genes and by real time PCR based on TaqMan® chemistry.
About 300 fg of bacterial DNA (75 bacterial cells or 525 copies of ribosomal DNA
genes) were consistently detectable. Three out of the nine RAPD-PCR decamers used to
type the sixteen samples of Salmonella typhi generated twelve distinct PCR types. A total
of 85 RAPD bands (81 polymorphic bands) were scored. The discriminatory indices of
the three primers ranged from to 0.508 to 0.675. By combining the profiles obtained with
the primer trio, an excellent discrimination index of 0.942 was attained. RAPD PCR
represents a fast method with high potentials in surveillance and epidemiological
investigations of Salmonella infections.