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العنوان
Comparative proteomic and genomic study between salmonella gallinarum and other salmonella serovars isolated from poultry /
المؤلف
Radwan, Moustafa Ismail.
هيئة الاعداد
باحث / مصطفى اسماعيل رضوان
مشرف / كاميليا محمود عثمان
مشرف / منى محرز على
الموضوع
Poultry. Salmonella.
تاريخ النشر
2009.
عدد الصفحات
151 Leaves :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البيطري
تاريخ الإجازة
1/1/2009
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - Bacteriology, Immunology and Mycology
الفهرس
Only 14 pages are availabe for public view

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Abstract

Comparative proteomics analysis of the cytosolic proteins of Salmonella Gallinarum (SG) and Salmonella Enteritidis (SE) isolated from poultry was performed. The 2-DE-separated proteins were compared between serovars via image master 2D Elite software and the identified spots that showed up or down regulation were selected for further identification using MALDI-TOF-MS. The differential expression among different serovars of the NCBI-matched proteins was further confirmed by Quantitative Real Time PCR.
The protein spots that were either constantly detected in all representative gels of SG and were completely absent in SE, or those spots that showed marked over-expression in SE over SG were selected for further characterization. A total number of 34 proteins were identified that representing diverse functional activities including energy production, metabolism, nucleic acid synthesis. Interestingly, some identified proteins have some relevance to bacterial.