الفهرس 
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Abstract
Infertility in buffaloes is considered as one of the most
important disorders which affects on the productivity and
reproductivity. The disease under concern in this study is Brucellosis in
Buffaloes. 102 blood samples and 17 tissue samples were obtained from
Buffalo cows from Governmental farm located at Ismaillia govemorate.
15 tissue samples were obtained from obligatory slaughtered cases from
Ossim slaughtered house in Giza governorate. All samples were
examined for brucellosis by using different diagnostic methods
including serological tests, isolation of Brucella organism, gross and
histopathological examination in the reproductive and lymph reticular
system, detection of Brucella antigen in tissue by
immunohistochemistry and finally detection of Brucella DNA in tissue
by Pf’R. The obtained results of serological tests were 16.6 0/0, 1l. 7 6 %,
12.74 %, 1l.76 % and 13.72 % with RBPT, TAT, MET, CFT and
ELISA respectively. Brucella was isolated from three out of thirty two
examined cases (9.37%). The most pronounced histopathological
changes were granulomatous, ulcerative and hemorrhagic endometritis
as well as granulomatous mastitis. Moderate to severe immuno-reactive
staining of brucella antigen were detected in supramanunary lymph
node, spleen and uterus. PCR assay of uterus and supramammary lymph
node gave positive PCR product (223bp) and (731 bp) using of Brucella
species primers and Brucella melitensis primers.