الفهرس | Only 14 pages are availabe for public view |
Abstract The present study was carried out to investigate: a) the different factors affecting IVM of camel oocytes and b) the ability of the in vitro matured camel oocytes to be fertilized in vitro using frozen-thawed camel semen. For this purpose, seven experiments were conducted. Experiment 1: Was carried out to study the effect of seasons of reproduction (breeding and non-breeding seasons) on oocyte yield and quality. Oocytes were collected using aspiration method. The oocytes yield in each season was recorded and the average number of oocytes /ovary was calculated. Oocytes were evaluated under stereomicroscope and classified as follows: Good quality: oocytes with five or more layers of complete cumulus cells and evenly granulated dark ooplasm. Fair quality: oocytes with 1-4 layers of cumulus cells and evenly granulated dark ooplasm. Poor quality: oocytes with cumulus cells incompletely surrounding the oocyte. Denuded: oocytes without cumulus cells and covered by zona pellucida. Experiment 2: Was carried out to study the effect of reproductive status of mature camels (pregnant and non-pregnant) on oocytes yield, quality and in vitro maturation. Oocytes were collected using aspiration method |