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Abstract E. coli strains were important causes of diarrheal disease in the world and remain a major public health problem of animals and human. The present investigation was carried out to evaluate the prevalence of E. coli among 180 samples from herbs, water and chicken and also control measures to prevent this growing health problem. It was aimed from this work to isolate, characterize and to evaluate the prevalence of E. coli serotypes, in addition to characteristics associated with haemolysin production, Congo Red binding activity, serum activity and antimicrobial susceptibility. In this study violet red bile lactose+ MUG, Eosin methylene blue, XLD, Hekton enteric medium, distinct biochemical tests and specific E. coli antisera were used for detection of E. coli strains. In this study 44 samples out of 180 sample was considered as E. coli with percentage 24.4%. In the present study it was recorded that 0125,0112 and 086 was the most prevalence serotype among the 44 isolated sample with percentages of 27%,25% and 18% respectively, followed by serotypes 0127, 0128, 0114, 044 and 0124with percentages of 1l.5, 7%, 4.5%, 4.5% and 2.5% respectively. All E. coli isolates had the ability to bind with C.R. dye giving red colonies (100%) and were sensitive to bactericidal effect of both human and sheep serum (100%). In other hand hemolytic test was done against 44 isolates and the results indicated that the isolates belonged to 0112 and 0114 serotype had hemolytic activity and the other serotype had no hemolytic activity. |