الفهرس | Only 14 pages are availabe for public view |
Abstract This study revealed that MDBK cell line gave a higher titer of the virus than HEP - 2 , Vero and BHK21 cell lines , so MDBK is the best cell line which can be used to propagate and study of some biological properties of BRSV and ORSVThe best time of harvestation of total yield virus for both viruses was found to be 126 hours post infectionBoth viruses were thormolabile and sensitive to Freezing and thawing cycles over 2 cyclesBRSV and ORSV are acid labile as their infectivity titre were dramatically affected by exposure to PH of 3 but maintained above PH of 4Complete virus inactivation was occurred at 7 and 6 hours for BRSV and ORSV respectively using BEI at 37\{u00B0\}CSkimmed milk was found to be the best stabilizer used in virus hyopholization for each virusThe application of polyclonal antiserum in the immunological reactivities with viral poly peptides in the western blot is not the ideal method to catch a significant difference between both bovine and ovine RSV |