الفهرس 
Cover PageOnly 14 pages are availabe for public view
 |  | from | 158 |  |  |
| | | from | 158 | | |
Abstract
The present study was carried out on 550 chickens & 1100 (tracheal and cloacal
swabs) representing 19 chickens farms (broilers-broiler breeders-layers) & 6 backyards
from different governorates in Egypt from 2006 ti II end of 2008 for detection of naturally
infection with highly pathogenic avian influenza HPAI H5 using Real-Time PCR
compared with immunoflurescent test. The RRT-PCR result showed that 17 positive flocks
and six positive backyards while two flocks were negative. On the other hand, RRT-PCR
recorded negative results for IL T & New Castle disease. The most severe histologic lesions
were shown in the upper and lower respiratory system that characterised by catarrhal to
fibrinous tracheitis, interstitial pneumonia with edema in the interstitial tissue. Severe
affection of blood vessels in different organs appeared in the form of congestion, fibrin
thrombi and vasculitis. The immunoflurscent test proved presence of AI viral antigen in
trachea, lungs, heart, intestine, pancreas, kidneys, ovary, oviduct and brain. The associated
pathological alterations in third year were affected by genetic diversity of HPAI H5N I
virus in the field.