الفهرس 
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Abstract
378 samples of animal origin were collected from 195 mastitic milk samples, 43 samples from lung tissues of slaughtered calves and 140 samples from the internal organs of diseased chickens. In addition, 191 samples of human origin were collected from 123 sputum samples from patients suffering from lung manifestations and 68 burn samples from patients exposed to third degree burns. Isolation and identification of P.aeruginosa (colonial morphology and biochemical characteristics) were done. Detection of the virulence factors( Congo red binding activity, Vero cells cytotoxicity and Enterotoxin assay using the infant mice) were performed, all the examined P. aeruginosa isolates were positive for Congo red binding and Vero cells cytotoxicity on the other hand, the enterotoxin activity was variable.
Antibiogram study for Isolates of P.aeruginosa also were done.
The genetic similarity and distance among the isolates were investigated using Random Amplified DNA Polymorphism (RAPD) , Amplified Fragment Length Polymorphism (AFLP). Plasmid profile was performed, P.aeruginosa isolates showed a degree of variation in plasmid numbers (from 1 plasmid up to 3 plasmids) and molecular weight (from 6.700 bp up to 23.130 bp). The data recorded showed enough similarity for some isolates and dissimilarity for other isolates . detection of multi-drug resistance to P. aeruginosa producing PER-1 Extended-spectrum β–lactamase (PER-1 ESBL) revealed that the gene appeared to be chromosomally located in some isolates.