الفهرس 
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Abstract
A. flavus strain after macroscopical and microscopical examination was used for production of aflatoxin B1 in yeast extract sucrose (YES) medium. Aflatoxin B1 was extracted from the liquid medium using chloroform and detected and quantitated using TLC. Aflatoxin B1 was used for production of aflatoxin B1-BSA conjugate that was used for immunization of Balb/c mice. Spleen cells were harvested from the hyper immunized mice to be fused with myeloma cell line (P3NS1) using polyethylene glycol 3000, 50% concentration as a fusogenic agent. The produced hybridomas were selected using HAT selective medium that was replaced by complete HT medium. from the 10th day after fusion, wells that contain colonies of hybridomas covering 30% or greater of the wells surface were screened for production of monoclonal antibodies against aflatoxin B1 using ELISA. 21 hybridomas were found to be reactive to aflatoxin B1. All were found to belong to IgG2a isotype except one was found to belong to IgM isotype.