الفهرس 
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Abstract
A total of 296 samples examined bacteriologically for Staphylococcus
aureus, 59 samples were positive for coagulase positive S.aureus, with a
total prevalence of 19.9 %. The prevalence among diseased chickens was
16.7 % While, 18.8%, 26.7 %, 12 %, 19.7 % of broiler farms, layerfarms, Giza
abattoirs and imported frozen chickens were positive, respectively. The high
percentage of coagulase positive S.aureus from diseased chickens was
isolated from joint (35.7 %) while, no positive results were found from
abscesses and eggs samples. The isolated strains of coagulase positive
S.aureus were tested for antimicrobial sensitivity. Most strains were resistant
to penicillin and ampicillin, less resistance was appeared to amoxicillin,
oxacillin, bacitracin, ceftrixone, methicillin, cephalothin and amoxicillin
clavulinic acid, while all strains were sensitive to vancomycin. On the other
hands ceftrixone, bacitracin and methicillin showed intermediate sensitivity
with the percentage of 40.7%, 25.4% and 20.3%, respectively. All isolated
strains of coagulase positive S.aureus were examined for the ~-Iactamase
production, 67.8 % were positive revealed from 54.5 %, 61.5 %, 70 %, 33.3 %
and 91.7 % from diseased chickens, broiler farms, layer farms, Giza abattoirs
and imported frozen chickens, respectively. Using PCR technique,
amplification of 310 bp fragment of mecA gene from the extracted DNA of
coagulase positive S. aureus isolates of different chicken samples and
related human resulted in PCR product in the percent of 10.2% (6/59); 15.4 %
(2/13) of broiler farms, 15 % (3/20) of layer farms and 8.3 % (1/15) of imported
frozen chickens were positive, while coagulase positive S.aureus isolated
from diseased chickens and Giza abattoirs samples were negative for mecA
gene. Regarding to the relation between the multidrug resistan, ~-Iactamase
production and presence of mecA gene on coagulase positive S.aureus
strains. The incidence of multidrug resistant S.aureus strains from human
(13/14; 92.9%) was higher than that of poultry (29/45; 64.4%). The incidence
of positive ~-Iactamase producing S.aureus strains from poultry (32/45;
71.1 %) was higher than that of human (8/14; 57.1 %), While, the incidence of
positive mecA gene from human was higher (3/14; 21.4 %) than that of poultry
(3/45; 6.7 %).