الفهرس | Only 14 pages are availabe for public view |
Abstract During the period of 2006 to 2008, 1096 blood samples collected from 92 chi c ken flocks were tested by HI test (21 of breeder flocks,63 of commercial layer flocks and 8 broilerflocks), collected from four governorates (Giza, Oalubia, Sharkia and Dakahlia)for evaluation of protective serological titer in surveyed vaccinated poultry flocks. In experimental study to evaluate the potency of 6 types of inactivated AI-vaccines (one H5N1 and five H5N2) administered at 7 days old chickens and to evaluate the potency of 2 types of inactivated AI-vaccines (one H5N1 and one H5N2) administered at 7 days old ducklings with full dose (0.5 cm sic), the results revealed that these vaccines were different, all give protective titer after where the obtained serological responses (3rQ week post vaccination) were differed in protective titer by HI test was done by using homologous and heterologous antigens. For the control of avian influenza, a rapid diagnosis by detecting the causative virus and identifying its subtype is essential. A rapid diagnosis kit for identification of influenza viruses by Rapid Antigen detection of three hundred samples collected from four governorates (Giza, Oalubia, Sharkia and Dakahlia), positive samples were 67, (22.33%). The aim of this study was to develop a rapid, cost-saving and effective method for influenza A virus subtype H5N1 detection. The selected primer set was used in single-step RT-PCR for simultaneous detection in multiplex format of the 276-, 189-, and 131-bp fragments, corresponding to sequences specific for M, H5 and N1 genes. The amplified DNA fragments were clearly separated by agarose gel electrophoresis. |