الفهرس 
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Abstract
Latency is a key aspect in BHV-1 life cycle like other alphaherpesvirus. Trigeminal ganglia (Tr.G.) of sensory neurons are the important target cells for BHV-1 and constitute a major site for latent infection. The aim of this study, elucidation the antiviral effect of genistein against BHV-1 in vitro on MOBK and In vivo in latently infected rabbit. BHV-1 was propagated on MOBK cells and titrated (105.4 TCI05ofml). Chemical extraction of isoflavones and determination of genistein concentration was done by use HPLC. The effect of genistein against BHV-1 in vitro was detected by inoculation of genistein in three steps, the first: it inoculated simultaneously with BHV-1, second: inoculation of BHV-1 on previously incubated genistein with MOBK cells, third: inoculation of genistein at 2, 4, 8, 12. 24h.p.i.of virus. In vivo latent infection of BHV-1 was established in white new Zeeland male rabbits inoculated with Colorado strain of BHV-1 intraconjunctivaly then followed by SIC injection of O.2mg of genistein. Induction of reactivation has been done by I/M injection of 4mg/kg/day OM. ocular samples are collected at 1 day up to 15 day p.1 & that collected at 3 &5 day post reactivation were examined serologically for BHV-1. Jjistopathological, serological examination and PCR analysis of tissue specimens (liver, lung, kidney, Tr.G.) collected directly after rabbit scarification revealed that 75% of rabbits treated with genistein even which treated also with OM show no virus shedding from Tr.G. Conclusion: genistein as a natural substance has antiviral effect against BHV-1 in vivo and invitro.