الفهرس 
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Abstract
In this study, 5 batches of inactivated RVF vaccine (ZH501) were
evaluated according to DIE RVF evaluation protocol through the
following tests:
1- Sterility test, it was done through culturing the tested vaccine on
specific media for aerobic, anaerobic bacteria, fungal and mycoplasma.
2- safety tests, to confirm the validity of the vaccine for animal used
without any post vaccinal reaction, through both vaccination of 10 mice
(3-5 days old) with 0.2 ml of tested vaccine then follow up for 10 days
and vaccination of 2 lambs with 5ml SIC and 5ml lIP then daily
recording of the body temperature for 10 days.
3- potency’ tests, to determine the duration of vaccinated animal
immunity till reach to the protective level by either by SNT and ELISA
through vaccination of 4 sheep with one the field dose 1ml SIC then the
sera sample were collected from the vaccinated sheep weekly till 7 week
post vaccination (WPV) or by EDso calculation by inoculation of 5 group
of 10 mice each of them by one dilution from 1:1 to 1:625 two doses one
week apart then challenged with virulent RVF virusl03 to 104 MIPLDso.
4- Using the Rt-PCR to confirm the efficacy of Azeridine as inactivator
to RVF virus used in tested vaccine through making RT -PCR on master
seed RVF virus as positive control samples, RVF virus after inactivated
with azeridine and on the eluted RVF antigen from the tested vaccine.